Bio::Restriction::EnzyUserMContributed PeBio::Restriction::Enzyme::MultiCut(3)NAMEBio::Restriction::Enzyme::MultiCut - A single restriction endonuclease
SYNOPSIS
# set up a single restriction enzyme. This contains lots of
# information about the enzyme that is generally parsed from a
# rebase file and can then be read back
use Bio::Restriction::Enzyme;
DESCRIPTION
This module defines a restriction endonuclease class where one object
represents one of the distinct recognition sites for that enzyme. The
method others stores references to other objects with alternative
sites.
In this schema each object within an EnzymeCollection can be checked
for matching a sequence.
REBASE report notation "Bsp24I (8/13)GACNNNNNNTGG(12/7)" means:
Bsp24I
5' ^NNNNNNNNGACNNNNNNTGGNNNNNNNNNNNN^ 3'
3' ^NNNNNNNNNNNNNCTGNNNNNNACCNNNNNNN^ 5'
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly address
it. Please include a thorough description of the problem with code and
data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
AUTHOR
Heikki Lehvaslaiho, heikki-at-bioperl-dot-org
CONTRIBUTORS
Rob Edwards, redwards@utmem.edu
COPYRIGHT
Copyright (c) 2003 Rob Edwards.
Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All
Rights Reserved. This module is free software; you can redistribute it
and/or modify it under the same terms as Perl itself.
SEE ALSO
Bio::Restriction::Enzyme, Bio::Restriction::Analysis,
Bio::Restriction::EnzymeCollection
APPENDIX
Methods beginning with a leading underscore are considered private and
are intended for internal use by this module. They are not considered
part of the public interface and are described here for documentation
purposes only.
new
Title : new
Function
Function : Initializes the enzyme object
Returns : The Restriction::Enzyme::MultiCut object
Argument :
others
Title : others
Usage : $re->others(@enz_obj_array);
Function : Stores auxiliary Enzyme::MultiCut objects for multicutting enzymes
Arguments : optional array of Enzyme::MultiCut objects
Returns : array of Enzyme objects
Added for compatibility to REBASE
purge_others
Title : purge_others
Usage : $re->purge_references();
Function : Purges the set of references for this enzyme
Arguments :
Returns :
perl v5.14.12011-07Bio::Restriction::Enzyme::MultiCut(3)
