Bio::SeqEvolution::DNAUsertContributed Perl DocuBio::SeqEvolution::DNAPoint(3)NAMEBio::SeqEvolution::DNAPoint - evolve a sequence by point mutations
SYNOPSIS
# $seq is a Bio::PrimarySeqI to mutate
$evolve = Bio::SeqEvolution::Factory->new (-rate => 5,
-seq => $seq,
-identity => 50
);
$newseq = $evolve->next_seq;
DESCRIPTIONBio::SeqEvolution::DNAPoint implements the simplest evolution model:
nucleotides change by point mutations, only. Transition/transversion
rate of the change, rate(), can be set.
The new sequences are named with the id of the reference sequence added
with a running number. Placing a new sequence into a factory to be
evolved resets that counter. It can also be called directly with
reset_sequence_counter.
The default sequence type returned is Bio::PrimarySeq. This can be
changed to any Bio::PrimarySeqI compliant sequence class.
Internally the probability of the change of one nucleotide is mapped to
scale from 0 to 100. The probability of the transition occupies range
from 0 to some value. The remaining range is divided equally among the
two transversion nucleotides. A random number is then generated to pick
up one change.
Not that the default transition/transversion rate, 1:1, leads to
observed transition/transversion ratio of 1:2 simply because there is
only one transition nucleotide versus two transversion nucleotides.
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to the
Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly address
it. Please include a thorough description of the problem with code and
data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of
the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
AUTHOR
Heikki Lehvaslaiho E<lt>heikki at bioperl dot orgE<gt>
CONTRIBUTORS
Additional contributor's names and emails here
APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
seq
Title : seq
Usage : $obj->seq($newval)
Function: Set the sequence object for the original sequence
Returns : The sequence object
Args : newvalue (optional)
Setting this will reset mutation and generated mutation counters.
set_mutated_seq
Title : seq_mutated_seq
Usage : $obj->set_mutated_seq($newval)
Function: In case of mutating a sequence with multiple evolvers, this
Returns : set_mutated_seq
Args : newvalue (optional)
rate
Title : rate
Usage : $obj->rate($newval)
Function: Set the transition/transversion rate.
Returns : value of rate
Args : newvalue (optional)
Transition/transversion ratio is an observed attribute of an sequence
comparison. We are dealing here with the transition/transversion rate
that we set for our model of sequence evolution.
Note that we are using standard nucleotide alphabet and that there can
there is only one transition versus two possible transversions. Rate 2
is needed to have an observed transition/transversion ratio of 1.
next_seq
Title : next_seq
Usage : $obj->next_seq
Function: Evolve the reference sequence to desired level
Returns : A new sequence object mutated from the reference sequence
Args : -
mutate
Title : mutate
Usage : $obj->mutate
Function: mutate the sequence at the given location according to the model
Returns : true
Args : integer, start location of the mutation, required argument
Called from next_seq().
perl v5.14.1 2011-07-22 Bio::SeqEvolution::DNAPoint(3)
